Sepharose activation

As member of the BioProcess resins range, NHS-activated Sepharose 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support Read less. Product specifications. Something went wrong - we were not able to load your agreed MSA pricing. Please try refreshing the page. Pack size All 25 mL 5 L mL. No reviews have been left for this product. Be the first to leave a review!

Looking for support assistance? If you can't find what you need, contact us to solve your problems. Approximately 2 mg of antibody can be coupled to 1 ml of swollen resin. CNBr-activated Sepharose will react with Tris buffer. It is important to remove any traces of Tris from the antibody solution.

Performing the reaction with antibodies in a buffer other than Coupling Buffer will reduce coupling efficiency. Extra CNBr resin can be prepared and incubated with coupling buffer lacking antibody to generate a negative control in later applications to test nonspecific binding to the CNBr support. Wash any unreacted antibody from the resin and then ensure that there are no unreacted CNBr sites remaining. The coupling efficiency can be calculated by dividing the total amount of antibody in the supernatant in Step 3 by the total amount of antibody loaded on the column in Step 2.

Author manuscript; available in PMC Feb Wilchek, M. CrossRef Google Scholar. Brummer, W. Solid-Phase Biochem. Volume Article Contents Abstract. Oxford Academic. Google Scholar.

Nobuko SENO. Select Format Select format. Permissions Icon Permissions. Abstract The optimal conditions for the activation of Sepharose by epichiorohydrin and subsequent immobilization of ligands were investigated. Issue Section:. You do not currently have access to this article. Download all slides. Sign in Don't already have an Oxford Academic account? You could not be signed in. Sign In Forgot password? Don't have an account?